This study was to evaluate the fungal inhibition activity of 5 isolates of Streptomyces spp.;LEF3, LEF6, LEF10, LEF11 and RDC12. Eight isolates of Colletotrichum musae isolated from banana could be used to fungal inhibition test with Streptomyces spp. by bioassay technique. Streptomyces isolates LEF11 showed the higheast potential to inhibit colony of all tested Colletotrichum musae on PDA at 4.0 mm,while Streptomyces LEF10, RDC12, LEF3 and LEF6 showed inhibition zone at 3.3, 2.7, 1.7 and 1.0 mm, respectively.